^（186）Re（Sn）－HEDP的制备及色层分析研究

PREPARATION OF ￣(186)Re(Sn)-HEDP AND THE COMPARATIVE ANALYSES OF CHROMATOGRAPHICAL METHODS

介绍￣（１８６）Ｒｅ（Ｓｎ）－ＨＥＤＰ的制备工艺，重点研究由ＳｅｐｈａｄｅｘＧ－７５和国产７１７型阴离子交换树脂组成的复合柱进行放化纯度分析的方法。用高压液相色谱对原料、纯化前后的￣（１８６）Ｒｅ（Ｓｎ）－ＨＥＤＰ作ＵＶ吸收和对应放射性峰比对分析，实验结果表明，ＨＰＬＣ色谱图与文献引用的￣（９９）Ｔｃ－ＨＥＤＰ、￣（１８６）Ｒｅ（Ｓｎ）－ＨＥＤＰ的ＨＰＬＣ色谱图基本一致，产品中杂质含量远比文献中的同类产品要少。原来采用的生理盐水和丙酮纸色层分析体系并不能进行放化纯度的分析测定，尤其是在还原不充分、标记率不高的情况下。而复合柱色层却能快速、简便地进行放化纯度的测定，并能对产品进行纯化。经动物实验证明，纯化的￣（１８６）Ｒｅ（Ｓｎ）－ＨＥＤＰ在动物体内分布较理想，小鼠的甲状腺、肝等器官的吸收比未经纯化的￣（１８６）Ｒｎ（Ｓｎ）－ＨＥＤＰ明显减少。

The labeling technology of ￣(186)Re(Sn)-HEDP is described, and a complex column(in SephadexG-75 and 717# resin)chromatographic analysis method is studied. Meanwhile, the comparative analyses, on the UV absorption and radioactive determination of purified ￣(186)Re(Sn)-HEDP and unpurified￣(186)Re(Sn)-HEDP are carried out by HPLC. The relative HPLC spectrums to be obtained are basicallycorrespondent with the spectrums of ￣(99)Tc￣m-HEDP、 ￣(186)Re(Sn)-HEDP quoted from reference, and contamination contents of our product are also less than that one. Experimental results demonstrate thatdouble-paper stripe chromatographic system are unapplicable to analysis radiochemical purity of labeledcompounds especially for the situations of unsufficient reduction of ￣(186)ReO_4 and low labeling rate. Thecomplex column chromatographic system used for the determination of radiochemical purity is both simple and rapid. Animal experiments in rats indicate that the radioactive absorption of thyroid and liverfor purified ￣(186)Re (Sn)-HEDP is obviously less than unpurified one.