血清总雌三醇的放射免疫测定

RADIOIMMUNOASSAY OF SERUM TOTAL ESTRIOL

用雌三醇（Ｅ＿３）－６－肟－ＢＳＡ为免疫原，制备了特异性强和亲和力高的抗血清。除了与雌二醇（Ｅ＿２）及雌酮（Ｅ＿１）的交叉反应率分别为０．２％和０．１５％外，和其它８种类固醇激素的交叉反应率均＜０．００１％。亲和常数Ｋ＿ａ＝２．７×１０￣（１０）ｌ／ｍｌ。用此抗血清和￣（１２５）Ｉ－６－Ｅ＿３－肟－碘代组胺建立了血清总雌三醇直接放射免疫分析法（ＲＩＡ）并测定１４７例不同孕周正常妇女血清总Ｅ＿３含量，结果满意。

The andsera of high specificity and fine avidity are prepared with estriol E_3-6-oxime-BSA as a im-munogen. The cross-reactivity of estradial(E_2 ) and estrone(E_1 )with the antiserum is 0.2% and0.15%respectively.The cross-reactivity of the other eight steroids hormones with the antisera are<0.0001%. Avidity constant of antiserum k_a is 2.7×10￣(10)l/mol.So,the direct RIA of serum total estriol is found with ￣(125)I-E_3-6-oxime-iodohistamine and antiserum,The contents of total estriol are determined for the normal women with different weeks of pregnancy.