聚酯型儿茶素对人肝癌细胞生长的抑制作用及其机制

The inhibitory effects on the growth of human hepatocellular carcinoma cell and its mechanism by theasinesin

目的 探讨聚酯型儿茶素对人肝癌SMMC 772 1细胞的生长抑制作用及其机制。方法 应用MTT法、集落形成试验、同位素掺入试验、细胞内cAMP/cGMP浓度测定、原位杂交等方法进行检测。结果 5 0mg·L-1聚酯型儿茶素对SMMC 772 1细胞的生长、集落形成有明显的抑制作用 ;[3H] TdR、[3H] Leu掺入试验证明其可明显抑制细胞DNA和蛋白质合成 ;聚酯型儿茶素作用后 ,SMMC 772 1细胞内cAMP及cGMP浓度明显增加 ,细胞的c myc基因mR NA水平表达明显降低 ,而 p5 3基因mRNA水平表达明显升高。结论 聚酯型儿茶素对肝癌SMMC 772 1细胞的生长有明显的抑制作用 ,此作用与其抑制细胞DNA和蛋白质合成、升高细胞内cAMP浓度和抑制c myc基因表达、增强p5

AIM To investigate the inhibitory effects on the growth of SMMC 7721 human hepatocellular carcinoma cell line and its mechanism by theasinesin. METHODS MTT assay,colony formation test, 3H TdR, 3H Leu incorporation test, determination of the intracellular cAMP and cGMP level, in situ hybridization were used. RESULTS Theasinesin at 50 mg·L -1 could significantly inhibit the growth and colony formation of SMMC 7721 cells. Theasinesin could strongly inhibit DNA and protein synthesis and increase intracellular cAMP levels. C myc gene expression was significantly decreased and p53 gene increased strongly in a dose dependent manner. CONCLUSION Theasinesin demonstrates significant inhibitory effects on the growth of SMMC 7721 cells. Its mechanisms may involve the inhibition of DNA and protein synthesis ,the elevation of intracellular cAMP levels, inhibition of c myc gene expression and enhancement of p53 gene expression.