合成鱼腥草素对脾切除动物细胞免疫功能的影响

The effect of Houttuyninum on cellar immunoiogic function in splenectomy animals

目的 研究合成鱼腥草素对脾切除动物细胞免疫功能的影响及作用机制。方法 建立小鼠、大鼠脾切除模型 ,测定迟发型超敏反应强度、外周血淋巴细胞ANAE阳性百分率、胸腺T细胞增殖能力、淋巴结T细胞增殖能力、淋巴结中淋巴细胞数量、外周血T细胞亚群。结果 合成鱼腥草素ig (6 0mg·kg-1,12 0mg·kg-1)能明显增强脾切除小鼠迟发型超敏反应强度 ,ConA诱导的淋巴结T淋巴细胞增殖能力 ,增加外周血淋巴细胞ANAE阳性百分率、淋巴结中淋巴细胞数量 ,而对胸腺T淋巴细胞增殖能力则无明显影响 ;合成鱼腥草素ig (4 0mg·kg-1,80mg·kg-1)明显升高脾切除大鼠Th 数量 ,降低Ts 数量 ,调节外周血T淋巴细胞亚群Th/Ts 比值。结论 合成鱼腥草素对脾切除动物细胞免疫功能具有调节作用 。

AIM To investigated the effect and mechanism of Houttuynium (HOU) on splenectomy animals. METHODS The models of splenectomy mice and splenectomy rats were made and delayed type hypersensitivity (DTH) reaction, ANAE (+) cell percentage of peripheral blood lymphocytes, Con A induced thymus and groin lymph node lymphocyte proliferation, the amount of lymphocytes of groin lymph node,and T cell subpopulation in peripheral blood were measured. RESULTS HOU ig (60 mg·kg -1 , 120 mg·kg -1 ) can strikingly enhanced delayed type hypersensitivity (DTH) reaction, ANAE (+) cell percentage of peripheral blood lymphocytes. strengthened Con A induced groin lymph node lymphocyte proliferation, raised the amount of lymphocytes of groin lymph node in splenectomy mice. But the effects of them on Con A induced thymus lymphocyte proliferation were not apparent. HOU ig (40 mg·kg -1 , 80 mg·kg -1 ) improved T h subset, reduced T s subset, and then modulated the rate of T h/T s in splenectomy rats. CONCLUSION HOU strengthened the cellar immune function of splenectomy animals by promoting the compensating capability of lymph node and regulating T cell subpopulation.