神经生长因子/胶源性神经营养因子基因修饰神经前体细胞单独和联合移植促进阿尔茨海默病模型鼠海马胆碱能纤维网的重建

IMPROVEMENT OF RECONSTRUCTION OF THE CHOLINERGIC FIBER NETOF HIPPOCAMPUS AFTER A SINGLE AND COMBINED TRANSPLANTATIONOF THE NEURAL PROGENITOR CELLS MODIFIED WITH NGF AND GDNF

目的 :观察神经生长因子 (Nervegrowthfactor,NGF) /胶源性神经营养因子 (Glialcellline derivedneurotroph icfactor,GDNF)基因修饰神经前体细胞 (Neuralprogenitorcell,NPC)单独和联合移植对阿尔茨海默病 (Alzheimer’sdisease,AD)模型鼠海马胆碱能纤维网的促重建作用。方法 :将NGF/GDNF基因修饰的NPC单独和联合移植入FF切断的大鼠侧脑室内。移植后三周取海马切片进行AchE纤维组织化学染色。结果 :CA1区和齿状回的NGF组、GDNF组和NGF +GDNF组的AchE纤维数分别为 3 2 %和 5 0 %、1 8%和 2 4 %、2 4 %和 5 8% ,明显高于损伤组(4 %和 6% )和NPC组 (7%和 9% ) ,P均 <0 .0 1 ;NGF组也高于GDNF组 (P <0 .0 5和P <0 .0 1 )。结论 :NGF/GDNF基因修饰的NPC单独和联合移植均能不同程度地促进AD模型鼠海马胆碱能纤维网的重建 ,其中NGF组和NGF

Objective:To investigate the improvement reconstruction of the cholinergic fiber net of hippocampus of AD rats after a single and combined transplantation of the neural progenitor cells (NPCs) modified with NGF and GDNF.Methods:The NPCs modified with NGF or GDNF were implanted in single and combined into the lateral cerebral ventricle after fibria fornix transection.The rats were killed 3 weeks after transplantation and the hippocampus sections were processed for AchE fiber histochemistry staining.Results:In the lesion side of the CA1 and dentate gyrus,there were 32% and 50%,18% and 24%,23% and 58% of AchE positive fibers in the NGF,GDNF and NGF+GDNF groups,which were significantly higher than that in the lesion (4% and 6%) and NPC (7% and 9%) groups ( P <0.01)respectively).Furthermore,the density of AchE positive fibers in the NGF group was also higher than that in the GDNF group ( P <0.05 and P <0.01 respectively). Conclusion:The cholinergic fiber net of the hippocampus and dentate gyrus can be rebuilt to different extent after a single and combined transplantation of the neural progenitor cells modified with gene of NGF and GDNF.The role of improvement reconstruction was greater in the NGF group and NGF+GDNF group than that in the GDNF group.