建立一种新的缺氧缺血小鼠模型

ESTABLISHING A NEW HYPOXIA-ISCHEMIA MODEL OF MOUSE

目的 :建立可靠的缺氧缺血动物模型。方法 :怀孕 2 1天小白鼠 ,剖腹后将含胎鼠的整个子宫置于 3 7℃水浴中 ,分别于水浴 1 5min、3 0min取胎鼠脑 (剖腹后立即取胎鼠脑作为空白对照 )。结果 :缺氧缺血 1 5min ,脑毛细血管内皮细胞吞饮小泡增多 ,部分星形胶质细胞脚板肿胀、血管周隙扩张 ,神经元无异常改变 ;缺氧缺血 3 0min ,毛细血管周围基膜样物质减少 ,血管周隙极度扩张 ,星形胶质细胞脚板大空泡样变 ,神经元显著肿胀 ;随着缺氧缺血时间的延长 ,脑含水量呈递增趋势 (P <0 .0 1 )。提示 :血脑屏障受损先于神经元 ,系血管源性脑水肿。对孕鼠实施“延迟剖宫产术” 。

Objective:To establish a novel hypoxia ischemia model of animal.Methods:The entire uterus including the foetuses were obtained from female mice on the last day of gestation for experiment,and were incubated in at 37℃,divided into one control group and 2 asphyctic groups (15 min and 30 min).The time of asphyxia was measured from the blood circulation and the uterus was cut off till completion of the cesarean section when the uterus was opened and the pups were taken out.Results:(1)Pinocytotic vesicles of endothelia increased and astrocytic foot swelled after 15 min of hypoxia ischemia.(2)Mitochondria of endothelia swelled,basement membrane of microvessels disappered and edema around capillaries after 30 min of hypoxia ischemia appeared.Conculsion:It is suggested that the damage of blood brain barrier is originated from vessels,and is earlier than neuronal death,and a novel animal model of asphgxia is established,providing a new way for the study of asphyxia of new borns.