人参皂苷诱导直肠癌细胞凋亡的临床观察

Clinical Study on Effect of Ginsenoside in Inducing Rectal Cancer Cell Apoptosis

目的 :观察人参皂苷对直肠癌细胞凋亡的影响。方法 :经病理诊断为直肠癌的患者 50例 ,随机分为两组。治疗组 35例 ,对照组 15例。治疗组术前每天用 84 5%的人参皂苷 4 0～ 60ml,保留灌肠 4～6h ,连续用药 6～ 8天。对照组用等量生理盐水同样方法灌肠。用药后手术切除肿瘤 ,术中取新鲜标本 ,送电镜检查。结果 :治疗组用药后 ,2 5例便频、便血、肛坠感减轻 ,占 71 4 % ,7例不全梗阻患者腹痛全部减轻 ;电镜观察 2 3例发现凋亡癌细胞 ,占 65 7%。对照组无上述改变。结论 :人参皂苷可诱导直肠癌细胞凋亡。

Objective: To explore the effect of ginsenoside in inducing rectal cancer cell apoptosis. Methods: Fifty patients of rectal cancer diagnosed by pathological examination were randomly divided into two groups, 35 in the treated group and 15 in the control group. The treated group received retention enema with 84.5% ginsenoside for 4-6 hrs every day, 6-8 days consecutively before surgical operation. The control group also received retention enema in the same way but with normal saline instead of ginsenoside. Fresh sample was taken during the operation and examined by electron microscopy. Results: Symptoms, such as frequent defecation, hematochezia and tenesmus, were palliated in most patients (25/35) and abdominal pain relieved in all the 7 cases of incomplete intestinal obstruction in the treated group. Electron microscopic examination showed that cell apoptosis was observed in cancer sample of 23 cases among the 35 cases of the treated group, amounting to 65.7%. While in the control group, above mentioned changes were not observed at all. Conclusion: Ginsenoside has the effect of inducing apoptosis in rectal cancer patients.