热毒清对HL-60细胞产生分泌型肿瘤坏死因子α及肿瘤坏死因子α转换酶mRNA表达的影响

Effect of Reduqing on HL-60 Cells in Secreting Tumor Necrosis Factor α Production and on TNFα Converting Enzyme mRNA Expression

目的 :探讨纯中药制剂热毒清抑制肿瘤坏死因子α(TNFα)失控释放的分子机制。方法 :采用细胞和分子生物学技术观察热毒清对HL 60细胞分泌炎性细胞因子———分泌型肿瘤坏死因子α(sTNFα)及肿瘤坏死因子α转换酶 (TACE)mRNA表达的影响。结果 :( 1) 1∶30稀释的热毒清能有效地控制内毒素脂多糖 (LPS)刺激所引起的sTNFα的高分泌。 ( 2 )热毒清单独作用HL 60细胞时 ,对TACEmRNA的表达虽无明显的抑制作用 ,但对LPS刺激增加表达的TACEmRNA具有明显的抑制效果。结论 :热毒清对LPS引起的炎性细胞因子sTNFα的分泌及对sTNFα激活的关键酶———TACE的基因表达具有双重抑制作用 ,提示热毒清可能是一种TACE良好的天然抑制剂。

Objective: To explore the molecular mechanism of inhibiting effects of Reduqing on the release of tumor necrosis factorα (TNFα). Methods: Using cytobiologic and molecular biologic technique to observe the effects of Reduqing on HL 60 cells in producing inflammatory cytokine secreting TNFα (sTNFα) and on mRNA expression of TNFα converting enzyme (TACE). Results: (1) Reduqing, diluted in ratio 1∶30, could effectively inhibit the increased HL 60 production of sTNF α induced by lipopolysaccharides (LPS); (2) Although no obvious effect on TACE was shown when Reduqing was applied alone on HL 60 cells, there was evident inhibitory effect of Reduqing on TACE mRNA expression enhancement induced by LPS. Conclusion: Reduqing could have the double inhibitory effects both on sTNF α production and on the gene expression of its key enzyme, i.e. TACE stimulated by LPS, suggesting that it might be a hopeful and excellent natural TACE inhibitor.