铜银离子协同氯化消毒对脊灰病毒核酸的破坏作用

Destructive effect of copper and silver ions with free chlorine on nucleic acid of polioviruses

目的 探讨铜银离子协同氯化消毒对病毒核酸的破坏作用 .方法 铜银离子协同氯化消毒 (4 0 μg· L- 1 银离子 ,40 0 μg· L- 1铜离子和 0 .3mg· L- 1游离氯 )前后用逆转录PCR的方法检测协同消毒前后脊灰病毒的核酸 ,并用免疫印记法 (DIBA)检测脊灰病毒的抗原性 (蛋白质 ) ;用核酸修复实验测定噬菌体 f2 的核酸修复率 ,并用血清学方法检测噬菌体f2的抗原性 .结果 RT- PCR检测显示铜银离子协同氯化消毒前脊髓灰质炎病毒 型 (PVI)的特异条带为阳性 ,协同消毒后的为阴性 ;DIBA检测显示协同消毒前后的结果均为阳性 .铜银离子协同氯化消毒灭活作用后 ,大肠杆菌噬菌体 f2的核酸修复率随作用时间延长而降低 ;噬菌体的抗原性与正常无明显差别 .结论 结果提示铜银离子协同氯化消毒灭活水中病毒的作用位点可能在病毒的核酸 .

AIM To study destructive effect of copper and silver ions in cooperation with free chlorine on nucleic acid of viruses. METHODS RNA of poliovirus was determined by using RT PCR and the antigenicity of poliovirus was detected by using dot immunobinding assays(DIBA) before and after synergistic disinfection.The repair rate of nucleic acid of col iphage was assayed by using nucleic acid repair experiment,and the antigenicity of coliphage was observed by usingserological reaction methods. RESULTS RT PCR showed that poliovirus I had a positive specific band before disinfection, and a negative one after synergistic disinfection; DIBA showed that positive results both before and after disinfection were positive results by DIBA.The experiment on repair of nucleic acid showed that the repair rate of nucleic acid of coliphage exposed to synergetic disinfection declined with time of action. Antigenicity of coliphage f 2 showed no significant difference before and after disinfection. CONCLUSION[WT5”,6BZ] During synergistic inactivation of coliphage and poliovirus by copper and silver ions in cooperation with free chlorine, the site of their inactivation is probably nucleic acid.