土生假丝酵母条件优化产类可可脂的研究

Optimum Condition in Prodyuction of Cocoa Butter Equivalent by Candida Humicolain

通过正交实验 ,得出土生假丝酵母产类可可脂的最佳培养条件为 :接种量 3 0ml;温度2 6℃ ;pH值 5 5;C/N比 60 ;培养时间 4d ;氮源为蛋白胨 ;碳源为蔗糖 (麦芽糖 ) ;铁元素 :FeSO4 ·7H2 O+FeCl3·6H2 O ,0 0 0 1g/l;ZnSO4 ·7H2 ) ,0 0 0 0 1g/l;MnSO4 ·4H2 O ,0 0 0 0 8;MgSO4 ·7H2 O ,1g/l;油酸 0 1g/l。在该条件下 ,土生假丝酵母的CBE得率为 10 2 % ,油脂系数为 5 6,其二位不饱和脂肪酸为93 4 %。

Through the orthogonality experiment,the optimization culture condition for accumulating CBE by Candida humicola was achieved as follwing;inoculum quantity,3.0ml,temperature,26℃;pH,5.5;C/N ratio,60;culture time, 4 d; nitrogen source ,peptone;carbon source,sultose(maltose);FeSO 4·7H 2O+FeCl 3·6H 2O,0 001 g/l;ZnSO 4·7H 2O,0 0001 g/l;MnSO 4·4H 2O,0 0008;MgSO 4·7H 2O,1 g/l;Oleic,0 1 g/l.Under such culture condition,we gain the Candida humicola CBE 10.2% from dry cell.the lipid coefficient is 5.6,the Sn 2 unsaturated fatty acid is up to 93.4%.