黄单胞冰核活性细菌发酵制备的初步研究

Studies on Fermentation of Ice Nucleation-active Xanthomonas campestris IN-1

应用正交设计与均匀设计对黄单胞冰核活性细菌的发酵制备条件进行了初步研究。结果表明 ,琥珀酸抑制了黄单胞菌生长与冰核活性的表达 ,甘油、蔗糖、乳糖、谷氨酸钠不同程度地促进了冰核活性和单胞菌的生长。较优培养基配方为酵母粉 1%、大豆蛋白胨 1%、MgSO4 ·7H2 O 0 0 5%、甘油 1%、蔗糖 2 %、乳糖 1%、谷氨酸钠 0 3% ,pH 7 0 ;较优发酵组合为细菌种龄 72h ,发酵时间 4

The fermentation of ice nucleation active bacteria (Xanthomonas campestris IN 1) was studied in this paper. The results showed that succinate in the growth medium had the negative function to the ice nucleation activity of Xanthomonas campestris IN 1, which was not consistent to the literature. Glycerol, sucrose, lactose and sodium glutamate could promote the ice nucleation activity and the cell growth. The optimum cultivation medium was presented as 1% of yeast extract, 1% of bacto peptone, 0.05% of magnesium sulfate, 1% of glycerol, 2% of sucrose, 1% of lactose, 0.3% of sodium glutamate, pH7.0. The optimum fermentation conditions were predicted as 72h of bacterial ages, 45h of fermentation time.