5′--AMP--QT_4亲和层析一步纯化乳酸脱氢酶

ONE-STEP PURIFICATION OF LACTATE DEHYDROGENASE BY 5′-AMP-QT_4 AFFINITY CHROMATOGRAPHY

进行了5′—AMP—QT_4亲和层析柱纯化乳酸脱氢酶(LDH)的试验。采用该方法一步即可从猪骨骼肌组织粗提液中获得高比活(566u/mg)的乳酸脱氢酶,酶活性回收率达85％以上。

An affinity column based on 5'-AMP-QT_4 was applied to purify lactate dehydrogenase (LDH). Using this method, LDH with high specific activity (566 u/rag)has been isolated from crude extract of porcine skeletal muscle by a single step. The rate of recovery of LDH activity was more than 85％.