非洲菊试管苗生根培养试验

Rooting of Gerbera janesonii Bolus tube shoots

非洲菊试管苗继代培养基中高质量浓度的细胞分裂素可抑制根的诱导 ;生根培养基中适量的生长素能促进根原基的分化和生长 ,蔗糖和无机盐质量浓度也影响生根的效果。以MS +BZ 1 0mg/L +IAA 0 .1mg/L培养基上增殖的芽在 1/ 2MS +IBA 0 0 1mg/L上培养 ,供试品种生根率在 15d内达均到 10 0 % ,且幼苗生长健壮 ,移出试管后经过适当的锻炼 ,成活率达到 10 0 %。以市售白糖和自来水代替蔗糖和去离子水 ,以河沙代替琼脂试验均取得了良好的效果。

This paper dealed with a rapid and useful technology for in vitro rooting of Gerbera janesonii Bolus tube shoots.The results indicated that the rooting medium containing IBA 0.1 mg/L after cultured in the multiplication medium containing above 1 mg/L was favorable to root pri mordia induction;1/2 MS is superior to 1/4 MS and IBA was better than NAA in rooting effect; 1/2 MS supplemented with IBA 0.01 mg/L gave the best rooting result with rooting ratio as high as 100% in half a month.The rooted plantlets were removed from culture vessels to plastic containers filled with soil,100% survival could be reached;better results were also gotten with sugar,tap water and river sand instead of sucrose,deionized water and agar.