火箭推进剂偏二甲基肼对大鼠γ-氨基丁酸受体的影响

Studies on acute toxicity mechanism of unsymmetrical dimethylhydrazine on γ-aminobutyric acid receptor

目的 研究偏二甲基肼急性中毒时大鼠γ 氨基丁酸 (GABA)受体的变化。方法 放射性配体结合试验。结果 在体外试验中 ,偏二甲基肼可降低GABA与大鼠脑GABA受体蛋白的结合 ,与对照组比较 ,差异有显著意义 (P <0 0 5 ) ;在体内试验中 ,偏二甲基肼染毒大鼠后 ,在惊厥发生前染毒大鼠脑GABA受体蛋白与GABA结合明显低于对照组 ,脑受体结合放射性计数为 40 8± 6 3,对照组为 80 9± 2 10 ;在惊厥发生时染毒大鼠脑GABA受体与GA BA结合明显高于对照组 ,脑受体结合放射性计数为 837± 10 0 ,对照组为 6 30± 141;两组相比 ,差异均有显著意义 (P<0 0 1)。结论 偏二甲基肼急性中毒时GABA受体的改变可能是其中毒机制之一。

Objective To study the changes of GABA receptor during the acute poisoning of unsymmetrical dimethylhydrazine(UDMH).Methods The binding of GABA and its receptor was measured with [ 3H]γ GABA binding assay.Results UDMH could decrease the binding of GABA and its receptor in rat brain significantly( P <0 05);UDMH could significantly decrease the binding of GABA and its receptor in UDMH treated\|rat brain before convulsion ( P <0 01);UDMH could significantly increase the binding of GABA and its receptor in UDMH treated\|rat brain during convulsion ( P <0 01).Conclusions Our results showed that UDMH could change the function of GABA receptor,it suggested that the change of GABA receptor may play an important role in the mechanism of UDMH acute poisoning.