犀黄丸(含药血清)对人肝癌细胞凋亡影响的形态学研究

The Morphological Study on the Induction of Apoptosis in Vitro by Chinese Herbal Medicine Xihuangwan

目的 :探讨中药复方犀黄丸对人肝癌细胞系 (bcl- 74 0 2 )凋亡的诱导作用。方法 :以血清药理学方法选择不同浓度的含药血清与人肝癌细胞系共同孵育不同时间后 ,应用倒置相差显微镜、荧光显微镜、透射电子显微镜对细胞生长状况和药物作用后细胞核形态进行观察。结果 :经含中药血清作用 2 4～ 72小时后 ,人肝癌细胞系出现体积缩小 ,部分细胞核破裂 ,呈现大小不等、形态不规则的荧光染色碎片或梅花状核型。染色质固缩 ,并凝结成块 ,聚集在核膜周边呈新月状或肾状。且凋亡细胞数量随含药血清的浓度增加和作用时间延长而增多。而对照组无上述形态改变。结论 :中药复方犀黄丸可诱导人肝癌细胞凋亡。

Purpose: To investigate the apoptosis induced by xihuangwan. Methods: Serologic pharmacologic method, phasecontrast, fluorescence, transmission microscope were used to observe the apoptosis with different concentrations of xihuangwan and different time. Results: The morphologic changes of apopptosis, such as condensed chromatin and apoptosis bodies were observed, and the number of apoptosis increased with the concentration of the xihuangwan and the time. No changes occurred in the control group. Conclusion: Xihuangwan could induce apoptosis in bcl 7402 hepatoma cells.