G-CSF减轻大鼠下肢缺血再灌注致肺功能损伤的实验研究

G-CSF Attenuates Pulmonary Dysfunction Induced by Ischaemia-Reperfusion of Lower Extremities in Rats

目的：研究重组粒细胞集落刺激因子（Ｇ－ＣＳＦ）对大鼠下肢缺血再灌注所致肺功能损伤的治疗作用。方法：雄性ＳＤ大鼠随机分３组，手术组及给药组麻醉后开腹解剖口主动脉．在肾动脉水平远端阻断１２０ｍｉｎ，开放、再灌注 １２０ ｍｉｎ；假手术组不阻断腹主动脉，余同手术组。３组均于术前经舌静脉注入伊文氏蓝（Ｅｖａｎ’ｓ ｂｌｕｅ ｄｇｅ） ３０ ｍｇ／ｋｇ；给药组术前经尾赢脉注入Ｇ－ＣＳＦ ２０μｇ／ｋｇ．余二组给予生理盐水１ｍｌ。术后取肺组织测定丙二醛（ｍａｌｏｎｒｌｄｉａｄｅｈｙｄｅ，ＭＤＡ）及伊文氏蓝含量。结果：给药组ＭＤＡ含量为（１．７１±０．３４）ｎｍｏｌ／ｍｇ，假手术组为（ｌ．７３±０．６５）ｎｍｏｌ／ｍｇ，均显著低于手术组〔（２．５４±０．３９）ｎｍｏｌ／ｍｇ］。 给药组伊文氏蓝含量为（１．５０±０．２９）μｇ／ｍｇ．假手术组为（０．１３±０．０７）μｇ／ｍｇ，均显著低于手术组［（（３．０７±１．１８）μｇ／ｍｇ］．结论：Ｇ－ＣＳＦ有减轻大鼠下肢缺血再灌注所致肺功能损伤的作用。

Objective: To investigate the therapeutic efficacy of Granulocyte colony-stimulating factor (G-CSF) against rat pulmonary dysfunction induced by ischaemia-reperfusion of lower extremities. Methods: Male Spague-Dawley rats were randomised into three group. The operated and G-CSF group underwent laparotomy and clamping of the infrarenal abdominal aorta for 120 min followed by 120 min of reperfusion; The sham-operated group underwent laparotomy for 240 min only; The G-CSF group was pretereated with GCSF 20 μg/kg through IV and the others were given 1 ml saline each. All groups received Evan's blue dye 30 mg/kg IV, preopenatively. Concentrations of lung malonyldiadehyde (MDA) and Evan's blue dye were measured as marker of lung injury. Results: Lung MDA level of G-CSF group was (1. 71 ±0. 34) nmol/mg; Sham-operated group was (1.73±0. 65) nmol/mg. both significantly lower than that of operated group [(2. 54±0. 39) nmol/mg]. Even's blue dye concentration was significantly higher in operated group [(3. 07±1. 18) μg/mg] than in G-CSF group ［(1.50±0.29) μg/mg］and sham-operated group [(0. 13±0. 07) μｇ/mg]. Conclusion: G-CSF has therapeutic efficacy against pulmonary dysfunction which was induced by ischaemia-reperfusion of lower extremities in rats.