摘要
目的:研究重组粒细胞集落刺激因子(G-CSF)对大鼠下肢缺血再灌注所致肺功能损伤的治疗作用。方法:雄性SD大鼠随机分3组,手术组及给药组麻醉后开腹解剖口主动脉.在肾动脉水平远端阻断120min,开放、再灌注 120 min;假手术组不阻断腹主动脉,余同手术组。3组均于术前经舌静脉注入伊文氏蓝(Evan’s blue dge) 30 mg/kg;给药组术前经尾赢脉注入G-CSF 20μg/kg.余二组给予生理盐水1ml。术后取肺组织测定丙二醛(malonrldiadehyde,MDA)及伊文氏蓝含量。结果:给药组MDA含量为(1.71±0.34)nmol/mg,假手术组为(l.73±0.65)nmol/mg,均显著低于手术组〔(2.54±0.39)nmol/mg]。 给药组伊文氏蓝含量为(1.50±0.29)μg/mg.假手术组为(0.13±0.07)μg/mg,均显著低于手术组[((3.07±1.18)μg/mg].结论:G-CSF有减轻大鼠下肢缺血再灌注所致肺功能损伤的作用。
Objective: To investigate the therapeutic efficacy of Granulocyte colony-stimulating factor (G-CSF) against rat pulmonary dysfunction induced by ischaemia-reperfusion of lower extremities. Methods: Male Spague-Dawley rats were randomised into three group. The operated and G-CSF group underwent laparotomy and clamping of the infrarenal abdominal aorta for 120 min followed by 120 min of reperfusion; The sham-operated group underwent laparotomy for 240 min only; The G-CSF group was pretereated with GCSF 20 μg/kg through IV and the others were given 1 ml saline each. All groups received Evan's blue dye 30 mg/kg IV, preopenatively. Concentrations of lung malonyldiadehyde (MDA) and Evan's blue dye were measured as marker of lung injury. Results: Lung MDA level of G-CSF group was (1. 71 ±0. 34) nmol/mg; Sham-operated group was (1.73±0. 65) nmol/mg. both significantly lower than that of operated group [(2. 54±0. 39) nmol/mg]. Even's blue dye concentration was significantly higher in operated group [(3. 07±1. 18) μg/mg] than in G-CSF group [(1.50±0.29) μg/mg]and sham-operated group [(0. 13±0. 07) μg/mg]. Conclusion: G-CSF has therapeutic efficacy against pulmonary dysfunction which was induced by ischaemia-reperfusion of lower extremities in rats.
出处
《海军总医院学报》
CAS
2001年第1期16-18,共3页
Journal of Naval General Hospital of PLA