人参皂甙对睡眠剥夺大鼠行为的影响

Effects of ginsenosides on behaviors of rats in sleep deprivation

目的 探讨人参皂甙 (ginsenosides,GS)对睡眠剥夺 (sleepdeprivation ,SD)大鼠行为的影响。 方法 用小平台水环境法 (flowerpot)建立大鼠SD模型。选用Sprague Dawley大鼠 2 4只 ,随机分为三组 :2 4小时SD组 ,48小时SD组和 72小时SD组 ,每组又设实验组和对照组。其中 ,实验组用GS连续灌胃 5天 ,对照组灌生理盐水 ,然后给予不同时间的SD观察各组SD前后“Y”迷宫和旷场反应中的行为变化。结果 ①“Y”迷宫学习 :2 4SD、48SD和 72SD的正确反应率 ,实验组显著高于对照组 (P <0 .0 5 ) ;触电时间 ,实验组显著低于对照组 (P <0 .0 5 )。这种差别随SD时间的延长而加大 ,72SD差别最为明显(P <0 .0 1)。②旷场反应测试 :72SD的中央格停留时间和垂直活动得分 ,实验组显著低于对照组 (P <0 .0 5 ) ;2 4SD和48SD ,二组无显著差异 (P >0 .0 5 )。 48SD和 72SD的水平活动得分 ,实验组显著高于对照组 (P <0 .0 5 ) ;2 4SD ,实验组和对照组无显著差异 (P >0 .0 5 )。这种差别也随SD时间的延长而加大 ,72SD差别最为明显 (P <0 .0 1)。结论 连续口服GS对SD造成的大鼠学习记忆能力受损有明显保护作用。在一定睡眠剥夺时间内 ,这种保护作用随SD时间的延长而增强。

Objective To explore the effects of ginsenosides(GS) on the behaviors of rats in sleep deprivation(SD). Methods SD was induced in male Sprague Dawlay rats by employing “flower pot”technique. 24 rats were divided into three groups randomly: 24SD(being deprived of sleep for 24 hours), 48SD(being deprived of sleep for 48 hours), 72SD(being deprived of sleep for 72 hours), and every group was also divided into two subgroups: experimental group and control group. Ginsenosides were forced fed to the experimental groups continuously for 5 days, and normal saline, to the controls, then the rats were being deprived of sleep for different time. Before and after SD immediately, the behaviors of rats were studied in 'Y' maze test and open field test. Results ①“Y”maze test. Compared with control groups, the accuracy of reaction of experimental groups increased significantly in 24SD, 48SD and 72SD(P<0.05), and the time of electrification, decreased markedly(P<0.05). The difference enhanced with the increase of SD time, and reached to the most in 72SD(P<0.01). ② Open field test. Compared with the control groups, the time of staying in center and the Rearing of experimental groups increased significantly in 72SD(P<0.05), and there was no obvious difference between 24SD and 48SD(P>0.05), the Crossing of experimental groups increased significantly in 48SD and 72SD (P<0.05), and there were no obvious difference in 24SD (P>0.05). Conclusion GS can improve the disorder of learning, memory and activity of rats induced by SD, and the effect may enhance with the increase of SD time to a certain extent.