肝癌组织HBV和HCV基因意义

CLINICAL SIGNIFICANCE OF HBV AND HCV GENOMES IN TISSUES OF HEPATOCELLULAR CARCINOMA

目的:探讨肝癌组织中HBV DNA和HCV RNA的表达。方法:应用原位聚合酶链技术检测了38例肝癌患者的肝癌及癌旁肝组织中的HBV DNA和HCV RNA表达。结果:肝癌组织中HBV DNA和HCV RNA的检出率为79％和68％,略低于癌旁肝组织的84％和71％(P>0.05);肝癌和癌旁肝组织中HBV DNA和HCV RNA双重感染率为47％和55％(P>0.05)。HBV DNA和HCV RNA在肝癌组织中以核阳性为主,而在癌旁肝组织中,HBV DNA以胞质阳性属多,HCV RNA以核质阳性为主。结论:成都地区原发性肝癌的发生与HBV和HCV感染密切相关,HCV在原发性肝癌发病中起着与HBV同等重要的作用。

Objective: To investigate the expression of HBV DNA and HCV RNA in liver cancer tissues and peri - tumor tissues. Method: Polymerase chain reaction in situ(PCRiS) was used to detect the HBV DNA and HCV RNA in paraffin - embedded liver cancer tissues and peri - tumor tissues from 38 liver biopsy samples of the patients with hepatocellular carcinoma( HCC) . Result: The positive rates of HBV DNA and HGV RNA in liver cancer tissues were 79% and 68% ,respectively,which was little lower than those in peri - tumor liver tissues(84% and 71% ,p > 0.05) . Double infected rates of both HBV DNA and HCV RNA were 47% in liver cancer tissues and 55% in peri - tumor liver tissues ( P > 0.05) . Both of these genomes in liver cancer tissues were mainly expressed in nuclei of the liver cells .But in peri - tumor liver tissues, HBV DNA existed mainly in the cytoplasm of the cells and HCV RNA in both of nuclei and cytoplasm of the cells. Conclusion: The development of hepalocellular carcinoma in Chengdu area was not only related to HBV infection, but also to HCV infection. It is suggested that HCV infection play an important role in the pathogenesis of hepatocellular carcinoma, similar to HBV infection.