用含药血清方法观察补肾益精方对破骨细胞功能的影响

The effects of Bushenyijing(invi gorating the kidney and supplementing th e essence of life)prescription on osteoc lasts resorption using drug sera method

目的 探讨补肾益精方含药血清的制备条件及其对体外培养破骨细胞骨吸收功能的影响。方法 取体重 2 70± 2 0g的SD大鼠 ,雌雄各半 ,每组 10只 ,同等条件下制备补肾益精方含药血清和对照血清 ,分别观察不同采血时间、不同喂药时间、不同灌胃剂量和血清添加量对骨吸收陷窝数量的影响。结果 末次灌胃后 1h采血组的骨吸收陷窝数明显少于其他各组 (P <0 0 5 ) ;灌胃 1d和 3d、7d组比较其含药血清的药效无差异 ,而与对照血清差异显著 ;随着灌胃剂量和含药血清添加量的提高 ,其骨吸收陷窝数明显减少。结论 补肾益精方含药血清对破骨细胞骨吸收功能具有明显的抑制作用 ;补肾益精方用于破骨细胞药效观察的大鼠含药血清制备条件为等效剂量连续两次 (间隔 2h)灌胃 ,末次灌胃后1h采血 ,其血清添加浓度为 30 %。

ObjectiveTo explore the preparation method of dru g sera of Bushenyijing Fang(BSYJF)(a pre scription of traditional Chinese medicin e)for invigorating the renal function an d the supplement of essence of life.Its effects on osteoclasts resorption tested in vitro were studied.Methods20 SD rats with average weight of 270±20 g,female and male in equal numbers,were divided into two groups randomly:the con trol group using sera and the group usin g sera including BSYJF.The effects of th e two kind of sera on the quantity of bo ne resorption lacuna were observed at di fferent blood sampling times,different m edicine administration times,different c onditions of medicine dosage and sera ad dition dosage.ResultsThe quantity of bone resorption lacuna a t one hour after last time of administra tion of medicine was much more smaller t han that of other groups(P<0 05[WT BZ ]).Durg effects showed no difference at 1st,3rd and 7th days after medication,bu t significient difference was seen in co mparison with the control sera.With medi cine dose and sera addition dose increas ing,the bone resorption lacuna decreased obviously.ConclusionThe drug sera of BSYJF significantly inh ibited osteoclast resorption.The prepara tion conditions of drug sera of BSYJF we re as follows:giving medicine of equival ent dose twice between 2 hours,sampling blood at one hour after last medication and adding sera to the concentration of 30%。