ANXⅠ在原发性肺癌及BEP2D转化细胞系中的表达(英文)

The Profile of ANX Ⅰ Expression in Lung Cancer and BEP2D Transformation Cell Model

[目的]体内、外的许多感染模型证实 ,ANXⅠ介导糖皮质激素的抗炎症效应 ,被认为是糖皮质激素抗炎症效应中的第二信使。然而 ,ANXⅠ与原发性肺癌之间的相关性尚未知晓。本文对此进行研究。[方法]采用免疫组化方法检测60例原发性肺癌标本中ANXⅠ的表达分布 ;蛋白组学方法描绘ANXⅠ在永生化、转化与恶性转化中的表达状况。[结果]ANXⅠ的表达分布与原发性肺癌的组织学分型相关 (P<0.05) ;在永生化细胞中表达丰度低 ,转化细胞较高 ,恶性转化细胞中最高。[结论]ANXⅠ可能参与原发性肺鳞癌形成的启动或促进阶段 ,对人支气管上皮细胞恶性转化具有较为重要的作用。

ANX Ⅰ has been convincingly demonstrated to mediate the anti inflammatory effects of glucocorticoids in a variety of models of inflammation in vivo and in vitro.It was thought to be one of the ‘second messengers' of glucocorticoids in the actions of anti inflamma_ tory.However,the correlation between ANX Ⅰ and primary lung cancer remains unknown.In this study,the expression distribution of ANXⅠwas examined in 60 primary lung cancer specimens by immunohistochemistry and in immortal,transformation and malignant transformation cell lines by comparative proteomics.The results showed that expression distribution of ANXⅠpresented a significant relationship with histological subtype of lung cancer (P<0.05).Low expression of ANXⅠexisted in immortal cells(989.13±59.34),moderate expression in transformation cells(1520.30±91.22),high expression in malignant transformation cells (2508.00±150.47,P<0.01).[Conclusions]These indicate that high expression of ANXⅠmay be involved in initiation or promotion stage of squamous lung carcinoma and play a critical role in malignant transformation of human bronchial epithelial cell as well.