聚酯型儿茶素的抗肿瘤及免疫调节作用

The Antitumor Activity and Immunomodulatory Effects of Theasinesin

目的：研究聚酯型儿茶素（ＴＳ）对小鼠移植瘤的抑制作用及对荷瘤小鼠免疫功能的影响。方法：应用小鼠艾氏腹水癌实体型、小鼠Ｓ１８０肉瘤和肝癌Ｈ２２３种模型进行了ＴＳ的抗肿瘤作用研究；应用小鼠迟发型超敏反应模型、小鼠碳粒廓清试验及脾淋巴细胞增殖试验研究了 ＴＳ对荷Ｓ１８０小鼠免疫功能的影响。结果：４００、２００、５０ｍｇ／ｋｇ ＴＳ对小鼠艾氏腹水癌实体型瘤生长有明显抑制作用；常规灌胃给药对小鼠Ｓ１８０和肝癌Ｈ２２的生长无明显抑制作用，但预防给药则对小鼠Ｓ１８０和肝癌Ｈ２２的生长有明显抑制作用，各组抑瘤率均大于 ３０％。５０、１００、２００ｍｇ／ｋｇ ＴＳ可使荷瘤小鼠降低的迟发性超敏反应恢复正常；亦可使荷瘤小鼠碳粒廓清指数 Ｋ和吞噬指数α值显著提高；还可明显增强荷瘤小鼠脾Ｔ淋巴细胞增殖反应。结论：ＴＳ对小鼠移植瘤有明显抑制作用，同时可增强荷瘤小鼠降低的免疫功能。

OBJECTIVE: To study the antitumor activity and immunomodulatory effects of theasinesin on mice transplanted tumor and tumor - bearing mice .METHODS: With three mice transplanted tumor models i.e. Ehrlich ascites tumor, sarcoma 180 and hepatic carcinoma hhz.we strdied the antitumor activity of theasinesin .The immunomodulatory effects of theasinesin on Smo~ bearing mice were measured by delayed type hypersensitivity(DTH), cleaning charcoal particles method, and splenocyte proliferation test .RESULTS: Theasinesin at doses of 400, 200, 50mg/kg could markedly inhibite the growth of Ehrlich ascites tumor(solid tumor) .Although theasinesin didn't have significant inhibitory effects on sarcoma 180 and hepatic carcinoma H22 in routine oral administration, the average inhibitory rates of each group,in manner of preventive administration, were all greater than 30% .Theasinesin at doses of 50,100,200mg/kg could significantly restore the decreased DTH in Siau180- bearing mice. It co-uld also markedly increase K, α and promote proliferation of activated T cells in S180- bearing mice .CONCLUSION:Theasinesin has significant inhibitory effects on mice transplanted tumors. It can also enhance the decreased immunological function on tu-mor- bearing mice.