摘要
目的:建立反相高效液相色谱法测定血清中大蒜素的浓度。方法:血清样品用10%三氯醋酸沉淀蛋白,正己烷提取后进样。采用Phenomenex C18(250mm × 4.6mm,5μm)柱;流动相:乙腈-1%冰醋酸(62:38,V/V),pH=3.5;流速:1.2ml/min,在室温下;波长:240nm检测。结果:该方法回收率为(99.2±6.6)%,最低检测浓度为0.18μg/ml,大蒜素血药浓度在0.29~12.4μg/ml范围内峰面积与浓度线性关系良好(r=0.9 977),日内 RSD<5.5%(n=5),日间 RSD<5.8%(n=5) 结论:本法快速、准确、灵敏,能较好满足大蒜素临床药代动力学研究的需要。
OBJECTIVE: To develope a reversed - phase high performance liquid chromatographic(RP - HPLC) method for the determination of allitridum in human serum .METHODS: The serum sample was injected after being deproteinized with trichloroaceticacid and being extracted with hexane.A Phenomenex Cis was used as the stationary phase and the solution being composed of acetonitrile -1% HAc(62 : 38,V/V)as the mobile phase with a flow rate of 1.2 ml/min.The serum samples were monitored at 240nm .RESULTS: The peak area of allitridum against allitridum concentration was linear with a correlation co-efficient of 0.9 977 over the range of 0.29- 2.4 μg/ml,the limitation of detection was 0.18 μg/ml. The average recovery was (99.2±6.6)%, within-day RSD< 5.5% (n = 5), between-day RSD<5.8% (n = 5) .CONCLUSIONS: This method is rapid, accurate and scnsitive.it is very suitable for the investigation on clinical pharmacokinetics of allitridum.
出处
《中国药房》
CAS
CSCD
2001年第4期226-227,共2页
China Pharmacy