美解眠诱发癫痫大鼠大脑皮质NO和SOD的变化研究

The change of nitric oxide and superoxide dismutase in cerebral cortex of the rat seizures induced by bemegrides

目的 观察癫痫大鼠脑中的一氧化氮 (NO)和超氧化物歧化酶 (SOD)的变化 ,并探讨 NO的氧化还原状态对癫痫的作用。方法 通过美解眠诱发致痫的大鼠模型 ,分别取癫痫发作时及癫痫发作刚停止时的大脑运动区皮质 ,匀浆后测定 NO的含量和 SOD活力。结果 癫痫发作组及癫痫发作刚停止组 ,大脑运动皮质的 NO含量均较正常对照组明显升高 ;癫痫发作组 ,脑内 SOD活力反而下降 ,癫痫发作刚停止组 ,脑内SOD活力明显升高。结论 癫痫发作组和短暂发作刚停止组 ,SOD活力 / NO含量比值具有显著差异 ,这些结果支持 NO的不同氧化还原状态在癫痫发作中起到不同的作用。

Objective To observe the change of nitric oxide(NO) and superoxide dismutase(SOD) in the epileptic rat brain cortex, and study the effect of the redox states of NO on epilepsy.Methods Using epileptic rat model induced by bemegride, the level of NO and the activity of SOD were measured in rat cerebral motor cortex during and only after seizures.Results The level of NO in rat cerebral motor cortex was significantly increased during and only after seizures than the normal controls. During seizures, SOD activity was decreased; but only after seizures, SOD activity was significantly increased.Conclusion The value of SOD activity than NO content had significantly difference in rat cerebral cortex between during and only after transient seizures. These results suggested that the different redox state of NO would play different role du ring the seizure.