利用分子标记确定从水稻3N×2N组合中得到的F_2群体稳定性

Identification of F_2 Population Derived from the Cross of 3N×2N in Rice Using RFLP and SSR Markers

同源三倍体株系 43是从水稻双胚苗株系 90 0 4群体中筛选出来的。用它作为母本和正常的二倍体粳稻品种“中丹 2号”杂交 ,得到了具有双亲性状的二倍体后代 ,收获其自交结实种子 ,次年获得一个表现稳定一致的F2 群体 ,编号“942”。为了证实这个群体稳定的真实性 ,利用RFLP和SSR 2种分子标记进行了验证。结果表明

Autotriploid strain 43 selected from twin-seedlings 9004 population was crossed with diploid japonica variety zhongdan 2. Some diploid F 1 plants were obtained and one stable F 2 population No. 942 was generated from the inbred F 1 seeds next year.To prove its stability,We employed the RFLP and SSR markers.The results showed that the F 2 population is uniform at molecular level.