栉孔扇贝核糖体DNA转录间隔子序列研究及其潜在应用(英文)

Sequence study and potential uses of ribosomal DNA internal transcribed spacers in scallop Chlamys farreri

以相应引物PCR扩增栉孔扇贝 (Chlamysfarreri)核基因组的核糖体DNA两个转录间隔子 (ITS - 1和ITS - 2 ) ,PCR产物经T载体连接后进行克隆、测序 ,分别得到了 340bp和 510bp的碱基序列 ,序列大小非常适合遗传变异及分子系统学研究。其A、T、G、C含量在ITS - 1分别为 32 .0 6 % ,2 0 .59% ,2 2 .35%和2 5.0 0 % ,在ITS - 2分别为 30 .0 0 % ,2 1.37% 2 4 .12 %和 2 4 .51%。这两个变异性较大的序列在扇贝种群中应用潜力很大 ,可广泛用于种内群体间遗传变异研究、种质鉴别及系统学研究。

The ribosomal internal transcribed spacer regions ITS-1 and ITS-2, extracted from mantle tissue of wild Chlamys farreri, were amplified via PCR using relevant primers. The PCR products were ligated into T-vector, then cloned and sequenced. Two nucleotide sequences were got with very suitable sizes of 340 bp and 510 bp for studies on genetic variation and phylogeny. The contents of A,T,G and C were 32.06%,20.59%,22.35% and 25.00% in ITS-1 and 30.00%, 21.37%, 24.12% and 24.51% in ITS-2. The primers of ITS-1 and ITS-2 proved to be very universal in a large variety of mollusc species. The potential uses of the 2 sequences for genetic variation studies and phylogenetic research in scallop species were discussed too.