红枣脱毒试管苗无性系建立的研究

Studies on the Establishment of the Vegetative Multiplication System of the Jujuba Des virus Test tube Seedlings

用狗头枣、掉牙枣和骏枣树一年生休眠枝 ,于 ( 55± 1 )℃热水浸泡 2 h后水培发芽 ,取 3个良种枣水培芽为外植体 ,经组培建立其脱毒试管苗无性系。适宜的初代培养基是 MS+ 6 -BA 1 .0 mg/L + NAA0 .1 mg/L(或 IAA 0 .1 mg/L;NAA 0 .1 mg/L+ IAA 0 .1 mg/L)。适宜的继代增殖培养基是 Cy I+ 6 -BA1 .4～ 1 .6 mg/L+ IBA0 .1 8～ 0 .3mg/L,可使培养的有效繁殖系数达 1 5.6 4～ 1 8.96 /30 d。已生产出试管苗 3万多株 ,为工厂化无毒枣苗的生产奠定了基础。另外 。

The 1 year old dormant branch of three fine cultivars of jujuba(Zizyphus jujuba Mill[Z.sativa]) of the Goutouzao, Diaoyazao and Junzao are immersed in the (55±1)℃ hot water 2 h, then it will sprouted in water culture.Taking 3 of its water culture buds as the explant then we have established the vegetative multiplication of des virus seedlings by tissue culture.The suitable medium of the primary culture is MS+6 BA1.0 mg/L+NAA0.1 mg/L(or IAA0.1 mg/L; NAA0.1 mg/L+IAA0.1 mg/L).The suitable subculture proliferating medium is CyI+6 BA1.4～1.6 mg/L+IBA0.18～0.3 mg/L,making propagation coefficient of subculture reached 15.64～18.96/30 d.By these we have produced more than 30,000 test tube more,there are also some discussion on the different culture varieties of the jujuba in this paper.