乌鸡调经丸对抗针刺引起的子宫平滑肌收缩的实验研究

Experimental Study on the Effect of “WU JI TIAO JING WAN" in Resisting Contraction of Uterine Smooth Muscle Caused by Acupuncture

本文观察了乌鸡调经丸对抗针刺“足三里”穴位引起的大鼠子宫平滑肌兴奋性收缩的作用和对由此引起的小鼠流产的保胎作用。用银针在大鼠“足三里”穴位处进针刺激 ,当子宫平滑肌兴奋达最高时 ,按 1 .0和 4.0 g生药 /kg口服乌鸡调经丸 ,再观察子宫平滑肌的兴奋状态 ;取受孕小鼠 ,每天按 1 .0 ,2 .0 ,4.0 g生药 /kg口服乌鸡调经丸 ,从第三天开始每天加针刺“足三里” ,连续 1 0天 ,共 1 3天。处死小鼠 ,打开腹腔检查各组小鼠是否有死胎。结果 :针刺“足三里”后 ,大鼠子宫兴奋性增加 ;小鼠发生流产数增多。而应用乌鸡调经丸后 ,大鼠子宫平滑肌明显松弛 ,引起流产小鼠数明显减少。提示乌鸡调经丸明显对抗针刺引起大鼠的子宫平滑肌兴奋 ,使子宫平滑肌松弛 ,促进小鼠的保胎作用。

Objective: To observe the effect of Chinese patent drug “WU JI TIAO JING WAN' (Bolus of Gallus gallus domesticus for Regulating Menstruation) in resisting acupuncture induced contraction of uterine smooth muscle to prevent abortion in rats and mice. Methods:① “Zusanli”(ST 36) was needled with the filiform needle twirled continuously for 10 min, once every 2 min. When the uterine smooth muscle contracted to reach the peak value, the rats were administered (ig) with “WU JI TIAO JING WAN' fluid at a dose of 1.0,4.0 g crude drug/kg, followed by observing changes of uterine smooth muscle again;② When got pregnant, the mice were given with (ig) “WU JI TIAO JING WAN' at a dose of 1.0,2.0,4.0 g crude drug/kg everyday. From the third day on, “Zusanli ”(ST 36) was punctured once everyday and continuously for 10 days. Then the mice were killed for examining the embryonic numbers. Results: When acupuncture stimulation was given, the rat uterine smooth muscle increased significantly in contraction; and the miscarriage mice also increased obviously in number. After administration of “WU JI TIAO JING WAN', the rat uterine smooth muscle relaxed remarkably and the abortion mice decreased in number considerably. Conclusion: “WU JI TIAO JING WAN' can resist acupuncture stimulation induced excitatory contraction of the rat uterine smooth muscle and lower the abortion rate in mice.