人巨细胞病毒抑制人巨核系祖细胞增殖的体外研究

Human cytomegalovirus inhibits the proliferation of CFU MK in vitro

目的 探讨人巨细胞病毒 (HCMV)对人巨核系祖细胞增殖的影响。方法 收集 2 0份脐血标本 ,采用巨核系祖细胞体外半固体培养技术 ,观察HCMVAD16 9株对巨核细胞集落形成单位 (CFU MK)生长的影响 ,分别用原位聚合酶链反应 (IS PCR)和RT PCR检测集落细胞中的HCMVDNA与即刻早期抗原 (IEA)mRNA。结果 HCMVAD16 9株在体外能明显抑制CFU MK生长 ,抑制程度与病毒感染滴度呈剂量依赖关系 ;经IS PCR检测发现病毒感染组CFU MK细胞中有HCMVDNA存在 ;RT PCR检测发现病毒感染组CFU MK细胞中有IEAmRNA的表达。结论 HCMVAD16 9株可直接感染巨核系祖细胞 ,并抑制其增殖与分化 ;

Objective To investigate the effect of human cytomegalovirus(HCMV) on the proliferation of colony forming unit megakaryocyte(CFU MK). Methods Semi solid CFU MK culture system was used to observe the effect of HCMV AD169 strain on CFU MK growth of 20 cord blood samples. HCMV DNA and immediate early antigen(IEA) mRNA in CFU MK were detected by in situ polymerase chain reaction(IS PCR) and reverse transcriptase polymerase chain reaction(RT PCR). Results HCMV AD169 suppressed the differentiation and proliferation of CFU MK in vitro significantly. The suppression was in a dose dependent fashion. HCMV DNA was successfully detected in colony cells from viral infection group, and did the expression of HCMV IEA mRNA. Conclusion HCMV AD169 can directly infect megakaryocyte progenitor and suppress their proliferation and differentiation.