RP-HPLC法测定解毒宣透汤中连翘苷、绿原酸及黄芩苷的含量

Determination of Forsythin, Chlorogenic Acid and Baicalin in Jieduxuantou Decoction by RP-HPLC

采用反相高效液相色谱法测定解毒宣透汤中连翘苷、绿原酸、黄芩苷的含量。色谱柱:Hypersil-C_(18)(5μm,200×4.6mm I.D.),连翘苷流动相为乙腈:水(21∶79,V/V),检测波长:277nm,平均回收率为97.0％,RSD=1.3％;绿原酸流动相为甲醇∶水∶冰醋酸(25∶74∶1,V/V/V),检测波长:320nm,平均回收率为96.1％,RSD=2.1％;黄芩苷流动相为乙腈∶水∶四氢呋喃(20.5∶77.5∶2,V/V/V,磷酸调至pH4),检测波长:274nm,平均回收率为98.5％,RSD=1.4％。本法操作简便,结果准确。

RP-HPLC was adopted in determination of forsythin,chlorogenic acid and baicate in Jieduxuantou decoction.