兔胸部火器伤后血浆肾上腺素和去甲肾上腺素的变化

Changes of plasma epinephrine and norepinephrine in rabbits with firearm thoracic wounds

目的 了解兔胸部火器伤血浆中肾上腺素 (epinephrine ,E)和去甲肾上腺素 (nore pinephrine ,NE )变化规律及在伤后应激反应中的作用。 方法 将 2 4只家兔随机分为 :单纯胸腔贯穿伤组 (A组 )、假致伤组 (B组 )和正常对照组 (C组 )。射击前A、B组右颈总动脉及右颈外静脉插管、C组耳中动、静脉头皮针穿刺接八道生理记录仪测压。伤前 5min、伤后 5 ,30min、1,3,6 ,12 ,2 4h取动脉血 2ml离心出血浆 ,采用高效液相色谱仪 (high -performanceliquidchromatography,HPLC)与电化学分析 (electrochemicaldetection ,ED)检测E及NE含量。 结果 (1)A组E及NE于伤后 30min达伤前 4.13和 3.45倍 ,分别于伤后 1,3h降至最低点 ,为伤前 2 .5 7和 0 .47倍 ,于 6h达第二高峰 ,为伤前 4.14和 3.6 8倍 ,2 4h后降至伤前水平 ;B、C组E及NE比较 ,差异无显著性意义 (P >0 .0 5 )。(2 )A组动、静脉压致伤瞬间 (2ms内 )升高 ,平均达伤前的 1.8和 6 .8倍 ,迅即下降至伤前水平 ,随后缓慢下降 ,分别于伤后 30 ,5min降至最低点 (伤前的 0 .6 3和 0 .2 5倍 ) ,之后缓慢回升 ,6 ,12h接近伤前水平 ;B、C组动、静脉压比较 ,差异无统计学意义 (P >0 .0 5 )。 结论 兔胸部火器伤后血浆中E和NE均显著升高 ;二者浓度变化主要源自创伤后失?

Objective To investigate the changes of plasma epinephrine (E) and norepinephrine (NE) in rabbits with firearm thoracic wounds. Methods A total of 24 rabbits were divided into 3 groups randomly, the simple thoracic penetrating injury group (Group A, n = 8), the pseudo-injury group (Group B, n = 8)and the normal control group ( Group C, n = 8). The pressures of carotid artery and jugular vein were recorded by eight-road physiological recorder at 5 minutes before injury. And the concentrations of plasma E and NE were detected through high-performance liquid chromatography (HPLC) and electrochemical detection (ED) at 5 minutes, 30 minutes, 1 hour, 3 hours, 6 hours, 12 hours and 24 hours after injury in all the rabbits. Results The concentrations of plasma E and NE in Group A were significantly higher than those of Group C (P<0.01) and changed regularly. There was no significant difference between Group B and Group C. The pressures of carotid artery and jugular vein of Group A increased by 1.8 and 6.8 times, respectively, during 2 millisecond after injury, then decreased to normal immediately. They decreased to the minimum (0.63 and 0.25 time, respectively) in 30 minutes and 5 minutes respectively and averagely, and raised to normal slowly after 6 hours and 12 hours respectively and averagely. There was no significant difference between Group B and Group C. Conclusions The concentrations of plasma E and NE increase significantly in the rabbits with thoracic firearm wounds. Their changes have close relationship with the severity of wound and can be used as a judging criterion during the early period of wound.