C57BL/6和BALB/C小鼠创伤反应差异性初步探讨

Preliminary study on posttrauma-response heterogeneity between C57BL/6 and BALB/C inbred mice

目的 观察C5 7BL/6和BALB/C小鼠对相同创伤的反应能力有无差异。 方法 采用冲击波致伤C5 7BL/6 (171只 )和BALB/C(15 9只 )近交系小鼠 ,观察两种小鼠伤后即刻 (0 )、1,6 ,2 4,72h的死亡率 ,对两种小鼠的肺损伤严重度进行病理形态学比较。 结果 在相同冲击伤条件下 ,C5 7BL/6和BALB/C小鼠伤后 72h内的死亡率和肺损伤程度比较 ,差异有非常显著性意义 (P<0 .0 1) ,其中C5 7BL/6表现出对冲击波具有较强的耐受能力 ,肺损伤程度也明显较BALB/C小鼠轻。两种小鼠内雌雄之间的死亡率和肺损伤程度比较 ,差异无显著性意义 ,并且绝大部分动物死亡发生在伤后 6h以内。 结论 C5 7BL/6和BALB/C小鼠对相同创伤的反应能力比较 ,差异具有显著性意义 ,这种差异性可能与它们在遗传背景上的差异有关。

Objective To observe the posttrauma-response heterogeneity between the C57BL/6 and BALB/C inbred mice. Methods The C57BL/6 and BALB/C inbred mice were injured by the same blast wave, and the mortality rates of both strains were analyzed at the points of 0, 1, 6, 24 and 72 hours after injury. The pathomorphological changes of both strains in the lung tissues were compared with each other. Results There were significant differences of the mortality rates and the severity of lung injury between the C57BL/6 and BALB/C inbred mice within 72 hours after blast injury. The C57BL/6 mice showed greater ability of resistance to the blast injury, and had milder lung injuries than the BALB/C mice. However, no significant difference of the mortality rate and the severity of lung injury was observed between the male and the female mice of both strains. Furthermore, we discovered that death of the mice almost occurred within 6 hours after blast injury. Conclusions There is significant heterogeneity of posttrauma-response between the C57BL/6 and BALB/C mice, which may be induced by the differences of their genetic background.