高胆红素血症新生大鼠闪光视觉诱发电位的实验研究

Experiment of Flash-Visual Evoked Potentials in Neonatal SD Rats Hyperbilirubinemia

目的 :探讨闪光视觉诱发电位 (F- VEP)与胆红素神经毒性的关系 ,为早期发现胆红素神经毒性提供理论依据。方法 :给 72只生后 7天的大鼠腹腔注射不同剂量的胆红素 ,制成高胆红素血症动物模型 ,观察其神经行为 ,检测 F- VEP。F- VEP检测后断头取脑 ,作冰冻切片 ,抽提脑组织胆红素。结果 :随着腹腔注射胆红素量的增加血清总胆红素浓度、脑组织内胆红素含量逐渐增加 ,F- VEP主波潜伏期逐渐延长 ,除 T1 组外余各组与对照组相比差异均有显著性 ;各实验组血清总胆红素浓度 4h、8h之间无差异 ,但脑组织内胆红素含量、F- VEP主波潜伏期除 T1 组外余各实验组 4h、8h之间相比有显著差异 ;血清总胆红素浓度与脑组织内胆红素含量无相关关系 ,脑组织内胆红素含量与 F- VEP潜伏期呈正相关 ;F- VEP反应高胆红素血症新生大鼠的胆红素神经毒性有较高的准确性。结论 :F- VEP可早期并较灵敏地反映胆红素的神经毒性。

Objective:To investigate the pathogenesis of bilirubin encephalopathy, expecially the correlation between Flash visual evoked potentials(F VEP) and bilirubin neurotoxicity. This study might provide the rationale to prevent bilirubin encephalopathy and its sequela with certain early meddling. Methods:Variant dosages of bilirubin were respectively injected into abdominal cavity of 72 neonatal SD rats of 7 days old, to prepare the hyperbilirubinemia animal model and observe their behavior. After anesthesia of the model rats F VEP were examined. The brain of model rats was perfused with normal saline. Its bilirubin level and frozen section were seperately determined and prepared. Results:In experimental animals the more increasing bilirubing was injected into abdominal cavity, the more brain bilirubin was raise, and the latencies of major wave in F VEP were delayed. There was significant difference in all the experimental groups, except T 1 group, compared with the control group. When we compared these datum on 4th h with those datum on 8th h, there were analog results in T 2～T 5 groups, but the serum bilirubin on 4th h and 8 th h had no similar difference in every experimental group. There was a positive relation between the brain bilirubing and the latencies of major wave, and no relation between the serum bilirubing and the brain bilirubin.Conclusion:We suggest that F VEP can early denote the neurotoxicity of bilirubin. This study might provide rationale with certain early meddling.