生物人工肾小管体外构建的研究

The Research of Equiping the Bioartificial Renal Tubule in Vitro

目的 :探讨体外构建生物人工肾小管 (RAD)的方法和 RAD重吸收钠离子 (Na+ )、肌酐 (Cr)、葡萄糖 (Glu)的功能鉴定。方法 :采用猪肾近曲小管上皮细胞株 (L L C- PK1)体外培养 ,然后植入 FH6 6血液滤过器内腔继续培养 ,体外制作模拟血液滤过加肾小管细胞的系统 ,即 RAD。测定本系统对 Na+ 、Cr、Glu的重吸收功能 ,并与未植入 L L C- PK1的滤过系统做对照。同时观察哇巴因、根皮苷对重吸收的特异性抑制作用。结果 :实验组 Cr重吸收率较对照组明显降低 (5 .9% :84.8% ,P<0 .0 0 1) ,Na+、Glu重吸收率大于 85 % ,且明显受哇巴因及根皮苷抑制 (重吸收率 <10 % ) ,祛除药物抑制因素后 ,重吸收率又能恢复 ,与阴性对照组比较有明显差异。结论 :本实验在体外成功构建 RAD。

Objective:To establish the method of bioartificial renal tubule assist device (RAD) and to investigate its reabsorption function in vitro.Methods:The cultivated LLC PK1 cells were seeded into the lumen of the hemofilter(FH66). We prepared the equipment to simulate the filtration in vitro, and to detect the function of RAD. The reabsorption rate of sodium,creatinine and glucose were measured.The effect of oubaine and phlorizin on the reabsorption rate of sodium and glucose was studied and compared with no LLC PK1 device. Results:The creatinine reabsortion rate of RAD was significantly decreased as compared with control group(5.9% vs 84.8%, P <0.001). The reabsorption of sodium and glucose was significantly inhibited by oubaine and phlorizin(reabsorption rate<10%),and can be recovered after oubaine and phlorizin were drived out.Conclusion:This method to equip RAD in vitro was successful and it had reabsorption function of sodium, creatinine and glucose.