星形细胞瘤恶性进展与P16蛋白表达

Malignant progress of astrocytomas and expression of P16 protein

目的 研究细胞周期负性调控蛋白在星形细胞瘤中的表达与肿瘤病理分级的关系 ,探讨纠正P16蛋白缺乏做为胶质瘤基因治疗策略的可行性。方法 SP免疫组化方法对 41例不同级别星形细胞瘤的P16蛋白表达进行观察 ,评价染色强度和阳性细胞百分数与星形细胞瘤恶性度的关系。结果 随着星形细胞瘤恶性度的增高 ,P16蛋白表达阴性例数增多 ,阳性染色细胞呈减少趋势。结论 星形细胞瘤P16蛋白表达水平与其分级呈负相关 。

Objective To research the relationship between the expression of cell cyclic negative control protein and pathologic grade in astrocytomas, to discuss the feasibility of using correct the scarce of P16 protein as genic treatment strategy in glioma. Methods An SP immunohistochemical staining technique was undertaken for detection the expression of P16 protein in 41 cases astrocytomas with various pathological grade, to evaluate the relationship between dying strength, precent of positive cell and the astromic maglinant degree. Results Negative expressional cases of P16 protein increased parallel with the elevation of astrocytomic malignant degree, and so decreased the positive dying cells. Conclusions This study suggests that the abnormal expression of negative interrelation between the level of astromic P16 protein expression and pathological grade in astrocytomas is an important factor which affect the occurence and development of astrocytomas.