大鼠脑缺血再灌注损伤IL-8变化的研究

The Change of the Content of Interleukin-8 after Cerebral Ischemia-Reperfusion Injury in Rats

目的 :探讨白细胞介素 - 8(interleukin - 8,IL - 8)在脑缺血再灌注损伤中的变化。方法 :采用改良ZeaLonga线栓法大鼠大脑中动脉闭塞 (middlecerebralarteryocclusion ,MCAO)模型 ,将大脑中动脉 (MCA)先行闭塞 2小时 ,然后进行再灌注不同的时间。应用双抗体夹心间接ELISA法检测实验组和对照组大鼠脑组织及血清中IL - 8浓度。结果 :缺血 2小时再灌注 1小时脑组织IL - 8含量较低 (P <0 .0 5 ) ,随再灌注时间延长逐渐升高 ,至 2 2小时达高峰 ,随后又降低 ;血清中IL - 8含量于再灌注 1小时最高 ,随后缓慢下降 ,再灌注 46小时降至对照组水平 ;空白组、假手术组脑组织内IL - 8含量高于缺血再灌注组 (P <0 .0 1)。结论 :IL - 8具有双重作用 ,既参与脑组织的正常代谢及生理功能 ,又参与了脑缺血再灌注损伤的病理生理过程。

Objective To study the changes of the content of interleukin-8 (IL-8) in cerebrum and blood after cerebral ischemia-reperfusion injury. Methods The middle cerebral artery (MCA) was occlude with modified Zea Longa's rat MCAO/R model. The MCA was occluded for 2 hours and reperfused for different periods. The conternt of IL-8 in brain tissue and blood serum was detected by double antibody sandwich enzyme-linked immunosorbent assay. Results The content of IL-8 in brain tissue was lowered at 1 hour after reperfusion ( P <0.05). It increased with reperfusion, peaked at 22 hours, and then went down. The concentration of IL-8 in serum peaked at 1 hour after reperfusion, and then decreased. It reached the level of the control group at 46 hours after reperfusion. The level of IL-8 in brain tissue in control group was higher than that in experimental group ( P <0.01). Conclusion IL-8 might possess double roles: involved in metabolism and function of normal brain tissue and involved in the pathophysiological course of cerebral ischemia-reperfusion injury as well.