壳聚糖对实验性脂肪肝大鼠肝及线粒体的体视学分析

Effects of chitosan on hepato-mitochondria in fatty liver rats by stereologic analysis

目的研究壳聚糖对实验性脂肪肝大鼠的防治作用及机理。方法采用低剂量CCl4 后肢注射 ,并高脂饮食复制大鼠脂肪肝动物模型 ,同时给予不同剂量的壳聚糖灌胃 ,通过计算机彩色图象分析及立体定量的体视学分析方法 ,测定脂肪肝大鼠肝脂变严重程度及线粒体超微结构的变化。结果 4g/ (kg·d)以上的壳聚糖可明显减轻脂肪肝大鼠肝脂变程度 ,改善肝细胞线粒体体视学参数 ,促进线粒体功能恢复。结论壳聚糖可通过受损线粒体结构功能的恢复 ,促进脂肪的氧化分解 ,而对脂肪肝的形成起到一定的防治作用。

PurposeThe aim is to study the preventing effect and mechanism of chitosan on fatty liver rats.MethodsThe rats given a low dose of carbon tetrachloride(CCl 4) and fed a high fat diet to induce fatty liver model were treated with chitosan in different concentrations.The extension of steatosis area and changes in mitochondrial ultrastructure in liver were estimated by a computer color image analysis system and a quantitative analysis(stereology).ResultsWhen rats were treated with a mid dose of chitosan[4g/(kg·d)],the degree of hepatocytic steatosis was markedly diminished, and the injured mitochondrial structure was improved compared with model group.ConclusionIt was suggested that chitosan might enhance the oxidative catabolism of lipid in fatty liver.