血脂变化对OLETF糖尿病大鼠肾小球细胞外基质成分含量的影响

Influence of plasma lipid level on the accumulation of glomerular extracellular matrix in OLETF diabetic rats

目的 采用 OL ETF大鼠来观察血脂变化对 2型糖尿病鼠肾小球细胞外基质含量的影响。方法 实验动物分为三组即正常对照组、糖尿病组和非诺贝特治疗的糖尿病组。非诺贝特 2 0 mg· kg- 1 · d- 1 灌胃 2 2周。用免疫组化的方法检测肾小球细胞外基质成分含量的变化。结果 糖尿病组血清总胆固醇、甘油三酯、极低密度脂蛋白和高密度脂蛋白水平明显高于正常对照 (P<0 .0 5 ) ,同时细胞外基质成分 ( 型胶原、层粘连蛋白、纤维连接蛋白 )含量亦较正常对照组增加 (三者 P<0 .0 1) ,降脂治疗后肾脏细胞外基质积聚明显减少 (与糖尿病未治疗组比 P<0 .0 5 )。结论 高脂血症可能与糖尿病肾小球硬化的发生有关 ,降脂治疗能够减缓肾脏的损害从而起到保护肾脏的作用。

Objective To investigate the effect of changes in plasma lipid levels on the accumulation of glomerular extracellular matrix in type 2 diabetic rats.Methods Rats were divided into three groups,namely,normal control,diabetes mellitus treated with Fenofibrate,which was gastrically administrated in the dose of 20mg·kg -1 ·d -1 for 22 weeks.A quantitative analysis of the components of glomerular extracellular matrix (ECM) were performed with immunoperoxide (ABC) method and the computer imagine analysis system.Results The result showed that diabetic rats had significantly higher plasma lipid levels and accumulation of ECM including collagen Ⅳ,laminine and fibronectin than the normal control ( P <0.01).22 weeks after the treatment with Fenofibrate,accumulation of ECM in diabetes treamtnent group was less marked as compared with the diabetes without treatment group ( P <0.05).Conclusion It is suggested that hyperlipidemia play a role in the pathogenesis of diabetic nephropathy.Lipid-lowering therapy may retard the progression of glomerulosclerosis in diabetes mellitus.