硫酸乙酰肝素蛋白多糖基因多态性与糖尿病肾病关系的研究

A study for HSPG gene polymorphism in Chinese type 2 diabetic nephropathy

目的 探讨硫酸乙酰肝素蛋白多糖基因 (HSPG)多态性与中国汉族人 2型糖尿病肾脏并发症之间的关系。方法 应用限制性内切酶 Bam HI的 PCR- RFL P法 ,检测 190例非 DM对照和 136例 2型糖尿病伴或不伴肾病者的 HSPG多态性基因型。结果 正常白蛋白尿组和异常 (微量和大量 )白蛋白尿组之间 Bam HI HSPG2等位基因频率和基因型频率无显著性差异 ,非 DM对照组和糖尿病组之间 Bam HI HSPG2等位基因频率无显著性差异 ,但基因型频率的差异有统计学意义。结论 中国汉族人 Bam HI HSPG2多态性与 2型糖尿病肾脏并发症的发生无显著性相关关系 。

Objective To explore the association between the heparan sulfate proteoglycan gene (HSPG) polymorphism and diabetic nephropathy in Chinese.Methods A case control study of 326 Chinese subjects including 136 type 2 diabetics with or without nephropathy and 190 non-diabetic control was performed.Genotype frequencies of HSPG2 polymorphism were studied by PCR-RFLP analysis with BamHI digestion.Results There was no difference in genotype frequencies or allele frequencies between normal albuminuria and abnormal albuminuria patients.Moreover,there was no association between diabetic patients and non-diabetic control in allele frequencies as well,but obvious difference in genotype frequencies(0.05> P >0.025) was found.Conclusion Our study showed the lack of association between HSPG polymorphism and diabetic nephropathy in Chinese type 2 diabetics.There may be association between genotype frequencies of HSPG polymorphism and diabetes in statistics.