IL-4对NOD小鼠1型糖尿病免疫干预及其机制的探讨

The immunotherapy of type 1 diabetes in NOD mice with IL-4 and the study of the possible mechanism

目的 探讨 IL - 4对 NOD鼠 1型糖尿病发病率、胰岛炎的影响及其机制。方法 采用人类 1型糖尿病动物模型 NOD(nonobese diabetic)鼠 ,IL - 4处理后检测血糖、尿糖及糖尿病发病率 ,HE染色观察胰岛炎程度 ,流式细胞仪测定周围及中枢淋巴细胞亚群的变化。结果 IL - 4处理组糖尿病发病率为 12 .5 0 % ,明显低于对照组 (6 2 .5 0 % ) (P<0 .0 5 ) ,且胰岛炎的严重程度亦低于对照组 ;处理组周围单个核细胞 CD+ 4 / CD+ 8及 IL - 2 R+ 细胞百分比增高 ,胸腺 CD+ 4 CD+ 8细胞百分比增高而 CD+ 4 CD+ 8细胞百分比下降 (P<0 .0 1)。结论 IL- 4有降低 NOD鼠 1型糖尿病发病率及胰岛炎严重程度的作用 ;这种作用可能与 IL-

Objective To study the effectiveness of IL-4 on type 1 diabetes in NOD mice and it's possible mechanism.Methods 4-week-old NOD mice,as a kind of type 1 diabetes model,were treated with IL-4 to investigate the incidence of insulitis,and lymphocyte subsets were determined by FACS.Results The incidence of diabetes in the IL-4 group was 12.50% which was obviously less than 62.50% in the control group ( P <0.05),and the degree of insulitis in the IL-4 group was also less than that in the control group.The lymphocyte subset in the IL-4 treated group showed higher CD + 4/CD + 8 cells and percentage of IL-2R + cells in spleen,and higher proportion of CD + 4/CD + 8 but decreased CD + 4/CD + 8 cells in thymus.Conclusion IL-4 can prevent NOD mice from developing type 1 diabetes,which may be related to the fact that IL-4 corrected the imbalance of immunoregulatory in NOD mice.