摘要
目的 研究阿片受体拮抗剂纳络酮 (Naloxone)对急性脑缺血损伤的保护作用机制。方法 线栓法制作Wistar大鼠局灶性大脑中动脉缺血 (MCAO)模型 ,应用逆转录聚合酶链反应技术测定各组脑组织匀浆白细胞介素 1β信使RNA(IL 1βmRNA)水平的变化。 结果 生理盐水治疗组IL 1βmRNA水平显著高于假手术组 (P <0 .0 1) ;纳络酮治疗组IL 1βmRNA水平显著低于生理盐水组 (P <0 .0 5 )。结论 阿片受体拮抗剂纳络酮可通过降低脑内IL 1βmRNA水平来减轻IL 1所致的神经功能损害 ,从而起到脑保护作用。
Objective To study the cerebral protective effect mechanism of naloxone in acute brain ischemic injury. Methods The rat focal cerebral ischemia models of middle cerebral artery (MCA) were established with the suture method. Using the technique of reverse transcription polymerase chain reaction (RT PCR),we detected the changes of Interleukin 1β messenger ribonucleic acid (IL 1β mRNA) level in brain tissue homogenate. Results The levels of brain tissue homogenate IL 1β mRNA in Saline treated group (ST) and Naloxone treated group (NT) were all significantly higher than that of Sham operated control group (SO) ( P < 0.01 ). Compared with ST group, the levels of IL 1β mRNA in NT group were significantly lower ( P < 0.05 ). Conclusion Administration of naloxone, an opioide receptor antagonist, has the brain protective effects in acute cerebral ischemia, by decreasing the level of IL 1β mRNA and reducing the acute cerebral ischemia injury caused by IL 1β.
出处
《卒中与神经疾病》
2001年第2期91-93,共3页
Stroke and Nervous Diseases
