摘要
目的 测定恶性疟原虫 -痘苗病毒重组活疫苗候选株免疫动物后产生白细胞介素 - 2 (IL- 2 )和干扰素 (IFN)的生物学活性水平。 方法 用 MTT法测定了其诱导的保护性细胞免疫反应 (Th1细胞免疫反应 )。 结果 用重组痘苗病毒在免疫家兔及大白鼠 4～ 6 wk后血清中 IL- 2的生物学活性增强 ,免疫后 6 wk家兔、大白鼠及小白鼠 3种动物血清中 IFN的生物学活性水平比免疫前明显升高。 结论 恶性疟原虫 -痘苗病毒重组活疫苗候选株免疫动物后可诱发机体产生
Objective To determine Th1 responses of the vaccinia virus vectored multi epitope live vaccine candidate for Plasmodium falciparum . Methods The sera of rabbit, rat and mice immunized with recombinant vaccinia virus were measured for the level of IL 2 and IFN by the method of MTT. Results There was obvious IL 2 activity in the sera of rabbit, rat and mice in 4-6 weeks after immunized with recombinant virus. The sera IFN level of those three kinds of animals in 6 weeks after immunization was significantly higher than that of pre immunization. Conclusion\ The vaccinia virus vectored multi epitope live vaccine candidate could stimulate Th1 responses in immunized animals.
出处
《中国寄生虫病防治杂志》
CSCD
2001年第1期1-3,共3页
Chinese Journal of Parasitic Disease Control
基金
联合国开发计划署 /世界银行 /世界卫生组织热带病研究培训特别规划处的资助
