微量法检测致病性外瓶霉对特比萘芬的敏感性

Evaluation of the antifungal susceptibility of Exophiala spp. against terbinafine by broth microdilution method

目的 : 探讨应用美国国家实验室标准委员会 (NCCLS)推荐的微量法检测致病性外瓶霉对特比萘芬的敏感性 ,为临床治疗由外瓶霉引起的暗色丝孢霉病提供依据。方法 : 参照M 2 7A方案(1997)检测 7种 6 6株外瓶霉最小抑菌浓度 (MIC) ,其中皮炎外瓶霉 (E .d) 19株、甄氏外瓶霉 (E .j) 18株、棘状外瓶霉 (E .sp) 12株、丛梗孢外瓶霉 (E .m) 13株、威尼克外瓶霉 (E .w) 2株、鲑鱼外瓶霉 (E .sa) 1株、嗜鱼外瓶霉 (E .p) 1株 ,菌悬液终浓度 (0 .5～ 2 .5 )× 10 3 CFU/ml,孵育温度 2 7℃ ,培养时间 5～ 7天。分别应用RPMI 16 40和SDB培养基比较其MIC值的差异。结果 : 特比萘芬对 6 6株外瓶霉的MIC范围为0 .0 0 4～ 0 .5 μg/ml,小于 0 .12 5 μg/ml的菌株有 5 8株 ,占 87.9% ,MIC50 分别为E .d 0 .0 3μg/ml,E .sp 0 .0 93μg/ml,E .j0 .0 3μg/ml。E .m 0 .0 3μg/ml,E .w 0 .0 12 μg/ml。应用RPMI 16 40和SDB培养基时MIC值具有一致性。结论 : 经过改良的M 2 7A方案可以用于特比萘芬对外瓶霉的药敏实验。致病性外瓶霉对特比萘芬的敏感性较高 ,由该类菌引起的暗色丝孢霉病可用特比萘芬治疗。

Objective:To evaluate the susceptibility of Exophiala spp. against terbinafine (TBF) according to the microdilution method proposed by NCCLS.Methods:The modified NCCLS M 26A was applieded. Sixty six strains were studied, including 19 of E.dermatitidis, 18 of E.jeanselmei, 13 of E.monilliae, 12 of E.spinifera, 2 of E.werneckii, 1 of E.salmonis, 1 of E.pisciphila.The strains were incubated at 27 ℃ for 5～7 days, with final inoculum size of 0.5～2.5×10 4CFU/ml. To compare the difference of susceptibility of Exophiala spp, media of RPMI 1640 and SDB were used during the culture, respectively. Results:The range of minimum inhibitory concentrations (MICs) was 0.004～0.5 μg/ml, and no resistant strains were detected. The MIC values of TBF to all strains were concordant when RPMI 1640 or SDB medium was used. Conclusion :The data indicated that the modified NCCLS M 27A method could be applied for antifungal susceptibility testing of TBF and Exophiala spp. which was sensitive to TBF. [