乳腺癌患者外周血T淋巴细胞rDNA转录活性分析

The studies of peripheral blood T lymphocyte cell rDNA transcription activity in patients with breast cancer

目的 通过乳腺癌患者外周血T淋巴细胞rDNA转录活性分析 ,探讨其对乳腺癌术后随访和预后监测的意义。方法 利用CIAS 10 0 0型细胞图像分析系统及相关的细胞培养、银染等技术 ,对 2 0例健康人、2 0例炎症患者和110例乳腺癌患者外周血T淋巴细胞rDNA转录活性进行分析 ,结果以核仁积分面积与细胞核积分面积的比值 (I .S % )和核仁银染积分光密度与细胞核银染积分光密度的比值 (I .O .D % )表达。 结果 炎症患者外周血T淋巴细胞的rD NA转录活性明显升高 ,乳腺癌患者外周血T淋巴细胞的rDNA转录活性明显降低 ,与健康人比较 ,差异有显著意义 (P<0 .0 1) ,转移和 /或复发时 ,T淋巴细胞rDNA转录活性进一步下降 (P <0 .0 5 )。

Objective To investigate the clinial significance of rDNA transcription activity of peripheral blood T lymphocytes in breast cancer patietns.Methods rDNA transcription activity of T lymphocytes was detected by CIAS 1000 cell image analysis system in 20 cases of health controls、20 patients with inflamationy and 110 patients with breast cancer.Results The rDNA transcription activity of peripheral blood T lymphocytes increased in inflammatory patietns and decreased in breast cancer paitents(P<0.01).There was a significant difference between postoperation group with recurrence or/and metastatic cancer patients(P<0.05).Conclusions The rDNA transcription activity of peripheral blood T lymphocytes may serve as a marker for following up and monitoring breast cancer.