肿瘤患者外周血NK细胞活性与sIL-2R水平的研究

Clinical significance of NK cell activity and serum soluble interleukin-2 receptor in cancer patients

目的 探讨肿瘤患者外周血NK细胞活性与血清sIL 2R的临床意义。方法 应用LDH释放法及双抗体夹心酶联免疫吸附试验 (ELISA)对 40例正常人、114例恶性肿瘤患者进行了外周血NK细胞活性和血清sIL 2R水平检测。结果 与正常对照组相比 ,肿瘤患者外周血NK细胞活性显著降低 (P <0 .0 1) ,血清sIL 2R水平明显增高 (P <0 .0 1) ;上述改变在伴转移患者中更明显 ,与不伴转移者相比 ,差异显著 (P <0 .0 0 1)。肿瘤患者外周血NK细胞活性与血清sIL 2R水平呈负相关 (r =-0 .473 ,P <0 .0 0 1)。治疗有效的转移癌患者NK活性明显增强 (P <0 .0 5 ) ,sIL 2R水平显著降低 (P <0 .0 1) ;无效者变化不明显 (P >0 .0 5 )。结论 血清中异常增高的sIL 2R可能是导致肿瘤患者NK细胞活性低下的原因之一。NK细胞活性和sIL 2R可作为研究肿瘤发生、发展及转移的重要免疫学指标 。

Objective To study on the expression and clinical significance of NK cell activity and serum soluble interleukin 2 receptor (sIL 2R) in cancer patients.Methods 114 cancer patients and 40 health controls were enrolled in the study.NK cell activity was measured by LDH released assay based on k 562 cells serving as target cells, and serum sIL 2R was measured by an enzyme linked immunosorbent assay (ELISA).Results NK cell activity of the cancer patients was decreased (P<0.01) and sIL 2R was increased (P<0.01) in comparison with that of healthy control,which was more commonly seen in those with metastasis than those without(P<0.001).There was a negative correlation between NK cell activity and serum sIL 2R level in cancer patients (r=-0.473,P<0.001).In those with metastasis who got complete or partial remission after treatment,the NK cell activity was significantly enhanced (P<0.05),and serum sIL 2R level was remarkably decreased (P<0.01).Conclusions It is suggested that the depressed NK cell activity may be a major consequence of the significantly elevated serum sIL 2R level in cancer patients.Both NK cell activity and serum sIL 2R can be considered as useful immunological parameters in studying the oncogenesis development and metastasis of cancer,and estimating the curative effect and prognosis for cancer patients.