米非司酮对人宫颈癌Hela细胞增殖及细胞周期的影响

Effects of Mifepristone on cell proliferation and cell cycle kinetics of human cervical carcinoma Hela cells in vitro

目的 探讨米非司酮对人子宫颈癌Hela细胞增殖及细胞周期分布的影响。方法 采用MTT测定 ,观察不同浓度米非司酮 (10、5、2 .5、1μmol/L)对Hela细胞增殖的抑制作用 ;通过ABC免疫组化染色及流式细胞仪分析 ,检测用药后Ki 6 7抗原、细胞周期分布及增殖指数的变化。结果 4个浓度的米非司酮对Hela细胞增殖均有抑制作用 ,并呈剂量依赖性 ,以 10 μmol/L浓度抑制作用最强 ,抑制率达 5 4% ;2 .5 μmol/L米非司酮可使Ki 6 7抗原表达明显减少 ,米非司酮 (5 μmol/L)使Hela细胞阻滞于G0 /G1期 ,不能进入S期及G2 /M期 ,抑制DNA的合成 ,使细胞增殖指数明显下降。结论 米非司酮体外对PR阳性的人宫颈癌细胞有明显的抑制作用 ,使细胞周期分布阻滞于G0

Objective To investigate the effects of mifepristone on cell proliferation and cell cycle kinetics of human cervical cancinoma cell line Hela in vitro.Methods The antiproliferation effects of four different concentrations of mifepristone(10,5,2.5,1μmol/L)on Hela cell line were detected with MTT assay.Immunohistochemcal technique ABC and flow cytometry analysis technique were used to determine the changes of Ki 67 antigen expression and cell cycle distribution and cell proliferation index after treatment with mifepristone.Results Four different concentrations of mifepriston inhibited the proliferation of Hela cells on a dose depending manner.The inhibition by mifepristone reached a plateau at 10μmol/L (54% inhibiton).mifepristone at 2.5μmol/L decreased the expression of Ki 67 antigen of Hela cells.It (5μmol/L)caused a decline in the proportion of cells in S and G2/M phase,blocked cell cycle progression in G0/G1 phase and reduced proliferation index(PI) of Hela cells.Conclusions In vitro,mifepristone significantly inhibits the growth of human cervical carcinoma cells with positive progestin recptor and arrests the cell cycle progression in G0/G1 phase.