胚胎脊髓移植后大鼠损伤脊髓c-Jun免疫反应的变化及其意义

Variation of c-Jun Immune Reaction of Injuried Spinal Cord in Rats after Fetal Spinal Cord Transplantation and Its Significance

目的 研究大鼠胚胎脊髓移植后能否影响c Jun免疫反应的表达和脊髓损伤后大鼠功能的恢复。方法 将动物分为脊髓半切洞损伤加胚胎脊髓移植组 (A组 )和单纯脊髓板切洞损伤加明胶海绵填塞组 (B组 )。每个时相点每组 6只动物 ,术后 1,3,7,14和 2 8天 ,应用行为学和电生理检查观察大鼠功能恢复情况 ,应用免疫细胞化学方法观察c Jun免疫反应的表达 ,采用计算机图像分析技术 ,进行定量分析。结果 大鼠脊髓损伤后c Jun免疫反应的表达A组明显高于B组 ,胚胎脊髓抑制后可使损伤脊髓高表达c Jun免疫反应持续到术后 7天。增加的c Jun免疫反应阳性细胞数目与神经功能的改善相平行。结论 胚胎脊髓移植后可使损伤脊髓高表达c Jun ,并促进大鼠功能恢复。

Objective To study the statement of immunoreaction of c Jun and functional recovery of spinal cord injury in rats after fetal spinal cord(FSC) transplantation.Methods Experimental rats were divided into two groups:spinal cord hemisection injury combination with transplants of FSC to injured site group(group A),and spinal cord injury that received gelfoam pledgets group(group B).On the lst,3th,7th,14th and 28th day after operation,6 animals in each group at each time phase,were observed for their function recovery with the methods of praxiology and electro physiological examination,the animals were then sacrificed for the observation of the statement of c Jun reaction by immnochemistry method and computer image analysis system was used for quantitative analysis.Results The c Jun immunoreaction of group A were significantly higher than that of group B.On the 28th day after injury,positive cells of group B could still be recognized and that of group A returned to normal level at 7 days after operation.These increases of GFAP positive cells were paralleled with a significant improvement in neurological function recovery.Conclusion FSC transplantation is sufficient to increase the statement of immunoreaction of c Jun and promote the recovery of neurological function of rats after spinal cord injury.