TAP1基因和FcεRIβ基因多态性与哮喘及其表型的相关性研究

The association analysis of TAP1 gene, FcεRIβ gene with asthma and its phenotype

目的 :为研究TAP1基因和FcεRIβ基因多态性与哮喘及其表型的关系。 方法 :选择TAP1基因中微卫星DNA标志———TAP1及FcεRIβ基因中RFLP位点———RsaI,采用Amp FLP和RFLP方法在散发哮喘病人中进行分析 ,并检测其血清总IgE水平 ,过敏原皮试及气道高反应性 ,应用相关分析观察TAP1及RsaI等位基因与哮喘及其表型的关系。结果 :RsaI位点等位基因片段与哮喘及气道高反应性相关 ,未见TAP1与哮喘或其表型相关。结论 :FcεRIβ基因可能是哮喘发病的一个候选基因 ,TAP1基因可能与哮喘的发病无关。

Objective:In order to known the relationship between TAP1 gene , FcεRIβgene and the development of asthma Methods:The microsatellite——TAP1 inside the intron of TAP1 gene and the RFLP locus inside the intron of FcεRIβgene were choiced to study in asthma patients using the Amp FLP and RFLP method Results:The allele of FcεRIβgene is associated with asthma and BHR, there is not relationship between TAP1 and asthma Conclusion:FcεRIβgene may be important candidate gene susceptibility to asthma TAP1 gene may not participate in the development of asthma