摘要
用生物化学方法 ,即用不同剂量的神经氨酸酶 (唾液酸酶 )作用相同的时间 ,和用相同剂量的神经氨酸酶作用不同的时间分别对红细胞进行处理 ,以达到不同程度地去掉其表面电荷。测量处理过的血液的粘度、血沉、红细胞聚集及各样本红细胞的DI、(DI) or、(DI) d 在不同切变率下的变形曲线 ,即DI γ、(DI)or γ和 (DI)d γ曲线及电泳率 ,并与正常对照组红细胞的相应参数及曲线作比较 ,发现两者之间的粘度、血沉、红细胞聚集及各种曲线存在明显差异。由此表明 ,红细胞表面电荷的多少直接影响血粘度、血沉及红细胞聚集与其红细胞变形性等流变特性 ,有力地证明了在低切变率下血液粘度与血沉主要反映红细胞的聚集行为 ,而在高切变率下的血液粘度则主要反映红细胞的变形行为。
We removed surface charge of RBCs in different degrees with biochemical methods, i.e, treating RBCs respectively with neuraminidase of different doses in the same period of time(1h) and of the same dose(75mu) in defferent periods of time. We measured the viscosity and sedimentation and the curves of DI , (DI) or and (DI) d and the electrophoretic mobility of all treated sample. Compared with the RBCs of control, the treated RBCs had obvious differences in their viscosities and sedimentations and electrophoretic mobility and three kinds of curves. It was indicated that the content of the surface charge of RBCs directly influenced blood viscosity and characteristics of microrheology. Therefore, it confirmed that the viscosity at low shear rate and the sedimentation of RBCs mainly reflected their behavior of aggregation and that the viscosity at high shear rate mainly reflected their behabvior of deformation.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2001年第2期152-157,共6页
Chinese Journal of Biomedical Engineering
基金
国家自然科学基金! (号 3 9770 2 0 3 )
博士点基金资助! (号 972 9)
关键词
神经氨酸酶
红细胞
微观流变特性
表面电荷
粘度
血沉
Neuraminidase
Erythrocyte
Microrheological characteristics
Surface charge
Viscosity
Sedimentation
