银屑病病人外周血及皮损组织T细胞亚群及IL-2R的变化

A STUDY ON THE CHANGES OF T LYMPHOCYTE SUBPOPULATION AND IL-2R IN TISSUE AND PERIPHERAL BLOOD OF PSORASIS PATIENTS

①目的 观察银屑病病人外周血与皮损组织中T淋巴细胞亚群及白细胞介素 2受体 (IL 2R)的变化 ,以探讨其与银屑病皮损形成的关系。②方法 采用碱性磷酸酶抗碱性磷酸酶法 (APAAP)和双抗体夹心ELISA法 ,检测了 5 7例银屑病病人外周血T淋巴细胞亚群和可溶性IL 2R(sIL 2R)的变化 ;采用免疫组化ABC法 ,检测37例银屑病病人皮损组织和非皮损组织及 2 0例正常皮肤组织的T细胞亚群CD+ 4,CD+ 8,CD+ 2 5的原位表达。③结果 银屑病病人外周血CD+ 4,CD+ 4/CD+ 8水平明显下降 ,sIL 2R ,CD+ 8水平上升 ,与对照组比较差异有显著性 (t =7.92～ 2 2 .16 ,P <0 .0 1) ;进行期CD+ 4,CD+ 4/CD+ 8,sIL 2R较静止期变化更明显 (t =5 .476 .95 ,P <0 .0 1)。银屑病皮损组织表皮及真皮CD+ 4,CD+ 8,CD+ 2 5细胞原位表达均显著高于正常组织及非皮损组织 (t =7.1117.36 ,P <0 .0 1) ;且进行期各指标表达较静止期明显 (t=2 .2 2 4.5 1,P <0 .0 5 )。④结论 银屑病皮损的形成可能与局部T淋巴细胞的活化有关。

Objective\ To explain the changes of T lymphocyte subpopulation and IL 2R in tissue and peripheral blood of psoriasis patients and the relation between them and psoriatic lesion.\ Methods\ The T lymphocyte subpopulation and sIL 2R of peripheral blood in 57 patients with psoriasis were determined by using APAAP and ELISA methods.The expression of CD + 4,CD + 8,CD + 25 in 37 psoratic lesion and non psoratic lesion,and 20 normal tissues were determined by ABC method.\ Results\ The levels of peripheral blood CD + 4, CD + 4/CD + 8 were lower,sIL 2R and CD + 8 were higher in the patients than those in the control (t=7.92-22.16,P <0.01);The changes of CD + 4, CD + 4/CD + 8,sIL 2R in developing stage were more significant than that in the static stage ( t=5.47-6.95, P <0.01).The expression of CD + 4,CD + 8,CD + 25 was higher in psoriatic lesion than that in non psoriatic lesion and normal tissues (t=7.11-17.36,P <0.01),and the indicators were more significant in developing stage than that in static stage (t=2.22-4.51, P <0.05).\ Conclusion\ psoriatic lesion formation might be related to the activation of T lymphocyte in the local skin . [