神经免疫肽对人多形核白细胞吞噬功能的影响

Effect of neuro—immunol peptides on phagocytosis of human polymorphonuclear leukocytes

用改良的NBT还原法观察了亮氨酸脑啡肽(LEK).甲硫酸脑啡肽(MEK)和P物质(SP)对正常人周缘血多形核白细胞(PMN)细胞吞噬杀伤作用的影响。结果表明:低浓度(10^(-10)mol/L)的三种神经免疫肽可使PMN的NBT阳性率分别提高到57.9%、49.8%和73.6%;而对照的阳性率为11.2%。表明三种神经免疫肽均能不同程度地增强正常人PMN的吞噬杀伤能力。不同神经免疫肽增强效应的差异可能是由于PMN的兴奋性和抑制性受体同时被激活后不同的净结果所致。

In this paper the effect of leucin enkephalin (LEK), met--enkephalin (MEK)and substance P(SP)on the phagocytosis of normal human peripheral PMN was studied with the improved NBT reducingmethod. Experimental results indicated that the three neuro--immunol peptides of low concentration(10^(-10) mol/L)raised NBT positive rates of PMN to 57. 9%, 49. 8% and 73. 6% respectively. Therate in the control group was 11. 2%. It showed that all of the three neuro--immunol peptides en-hanced phagocytosis of normal human PMN to varying degrees. The differences reflect the net resultsof simultaneous activation of the excited and inhibited type receptors of PMN.